«Detecting viruses early in an infection before symptoms appear, or from field samples, is difficult because the concentration of the viruses could be very low — often below the threshold of current detection methods," said Mauricio Terrones, professor of physics, chemistry, and materials science and engineering at Penn State, and one of the corresponding authors of the research.
«Early detection is important because a virus can begin to spread before we have the ability to detect it. The device we have developed allows us to selectively trap and concentrate viruses by their size — smaller than human cells and bacteria, but larger than most proteins and other macromolecules — in incredibly dilute samples. It further increases our ability to detect small amounts of a virus by more than a hundred times.»
The research team developed and tested a small, portable device that increases the sensitivity of virus detection by trapping and concentrating viruses in an array of carbon nanotubes. Dilute samples collected from patients or the environment are passed through a filter to remove large particles such as bacteria and human cells, then through the array of carbon nanotubes in the device. Viruses get trapped and build up to usable concentrations within the forest of nanotubes, while other smaller particles pass through and are eliminated. The concentrated virus captured in the device can then be put through a panel of tests to identify it, including molecular diagnosis by polymerase chain reaction (PCR), immunological methods, virus isolation, and genome sequencing.
«Because our device isolates and concentrates viruses purely by size, we can capture viruses that we don’t know anything about biologically — we don’t need any antibody or other molecular label," said Terrones. «Once we capture and concentrate the virus, we can then use other techniques such as
«Most lethal viral outbreaks in the past two decades were caused by newly emerging viruses. This
Viruses — such as influenza, HIV/AIDS, Ebola, and Zika — can cause sudden, unpredictable outbreaks that lead to severe
«We developed the technology to grow a forest of nanotubes and we can control the distance between the trunks," said Zheng. «The intertube distance can range from about 17 nanometers to over 300 nanometers to selectively capture viruses. The unique properties of the
The researchers validated the ability of their newly developed device to capture viruses from dilute samples using known concentrations of previously identified viruses as well as field samples of emerging and unknown viruses. «We developed a portable platform to enrich and isolate viruses based on their physical sizes," said
In addition to Terrones, Zheng, and Yeh, the research team at Penn State includes Yi Tang, Aswathy Sebastian, Archi Dasgupta, Nestor
The research was funded by the
