Developers
Joana S. Barbosa, Rossella Di Giaimo, Magdalena Götz and Jovica Ninkovic
Description of the technology
Adult neural stem cells (aNSCs) in zebrafish produce mature neurons throughout their entire life span in both the intact and regenerating brain. An understanding of the behavior of aNSCs in their intact niche and during regeneration in vivo should facilitate the identification of the molecular mechanisms controlling
In the frameworks of this technology was developed a protocol for labeling and repetitive imaging of aNSCs in vivo. Single aNSCs were labeled, allowing nonambiguous
Practical application
The technology can be further applied to study molecules and pathways that maintain the behavior of adult neural stem cells in the intact or the damaged brain in vivo, and to test compounds that can potentially modulate this behavior in vivo. This technology is highly promising for a use in experimental investigations in the fields of neuroscience, regenerative medicine, study of ageing processes, etc. The principles, supporting this technology, can be translated to animal models in other species.
Laboratories
- Institute of Stem Cell Research, Helmholtz Center Munich, Neuherberg/Munich (Germany)
- Center for Neuroscience and Cell Biology, University of Coimbra, Coimbra (Portugal)
- Department of Biology, University of Naples Federico II, Naples (Italy)
- Biomedical Center, University of Munich, Munich (Germany)
- Excellence Cluster of Systems Neurology SYNERGY, Ludwig Maximilian University of Munich, Munich (Germany)
Links
http://www.nature.com/nprot/journal/v11/n8/full/nprot.2016.077.htmlPublications
- Barbosa, J.S. et al. «
Single-cell in vivo imaging of adult neural stem cells in the zebrafish telencephalon." 11 Nature Protocols (2016): 1360–1370. - Barbosa, J.S. et al. «Live imaging of adult neural stem cell behavior in the intact and injured zebrafish brain." 348 Science (2015): 789–793.
- Baumgart, E.
v. et al. «Stab wound injury of the zebrafish telencephalon: a model for comparative analysis of reactive gliosis." 60 Glia (2012): 343–357.