Developers
Martin
Description of the technology
Ageing results in loss of tissue homeostasis across taxa. Particularly, in the intestine of Drosophila melanogaster, ageing is correlated with an increase in intestinal stem cell proliferation, a block in terminal differentiation of progenitor cells, activation of inflammatory pathways, and increased intestinal permeability. However, causal relationships between these phenotypes remain unclear.
The authors demonstrated that ageing results in altered localization and expression of junction proteins in the posterior midgut of Drosophila melanogaster, which is quite pronounced in differentiated enterocytes at tricellular junctions. Acute loss of the tricellular junction protein Gliotactin in enterocytes results in increased intestinal stem cell proliferation and a block in differentiation in intestines from young flies, demonstrating that compromised tricellular junction function is sufficient to alter intestinal stem cell behaviour in a
Thus, the investigation of specific tricellular junction function, localization and expression of junction proteins can serve as a technology, which helps to reveal the
Practical application
The study of
Furthermore, if
Laboratories
- Department of Molecular, Cell, and Developmental Biology, University of California, Los Angeles (USA)
- Department of Integrative Biology and Physiology, University of California, Los Angeles (USA)
- Molecular Biology Institute, University of California, Los Angeles (USA)
- Eli and Edythe Broad Center of Regenerative Medicine and Stem Cell Research, University of California, Los Angeles (USA)
Links
http://www.nature.com/ncb/journal/v19/n1/full/ncb3454.htmlPublications
Resnik-Docampo , M. et al. «Tricellular junctions regulate intestinal stem cell behaviour to maintain homeostasis." 19 Nature Cell Biology, (2017): 52–59.- Jones, D. L. & Rando, T. A. «Emerging models and paradigms for stem cell ageing." 13 Nat. Cell Biol. (2011): 506–512.
- Rera, M., Clark, R. I. & Walker, D. W. «Intestinal barrier dysfunction links metabolic and inflammatory markers of aging to death in Drosophila." 109 Proc. Natl Acad. Sci. USA, (2012): 21528–21533.