Developers
Kiwamu Takemoto, Hiroko Iwanari, Takeharu Nagai, Takao Hamakubo, Takuya Takahashi, etc.
Description of the technology
The synaptic delivery of neurotransmitter receptors, such as GluA1 AMPA (α-amino-3-hydroxy-5-
Authors developed a technique to inactivate synaptic GluA1 AMPA receptors using
GluA1 homomeric receptors are involved in
This method gives the valuable tool for future to study and manipulate complex behavior processes, such as memory, learning, etc, which serve the basis of complicated and
Practical application
The optical technique for inactivating synaptic proteins will enable elucidation of their physiological roles in cognition. The used approach provides a useful tool for elucidating not only the roles of GluA1 AMPA receptors, but also other synaptic proteins in cognitive function in various brain areas as well as in individual spines.
The technology can be applicable for study and modelling of memory changes in physiological conditions and diseases, as well as in process of transformations in organism with the course of time, for example, during ageing.
Laboratories
- Yokohama City University Graduate School of Medicine, Department of Physiology, Yokohama (Japan)
- Precursory Research for Embryonic Science and Technology, Japan Science and Technology Agency, Kawaguchi, Saitama (Japan)
- The University of Tokyo, Department of Quantitative Biology and Medicine, Research Center for Advanced Science and Technology (RCAST),
Meguro-ku , Tokyo (Japan) - Osaka University, The Institute of Scientific and Industrial Research, Ibaraki, Osaka (Japan)
Links
http://www.nature.com/nbt/journal/v35/n1/abs/nbt.3710.htmlPublications
- Takemoto, K. et al. «Optical inactivation of synaptic AMPA receptors erases fear memory." 35 Nature Biotechnology, (2017): 38–47.
- Takemoto, K. et al. «SuperNova, a monomeric photosensitizing fluorescent protein for
chromophore-assisted light inactivation." 3 Sci. Rep. (2013): 2629. - Takahashi, N. et al. «Locally synchronized synaptic inputs." 335 Science, (2012): 353–356.