Developers
Antonia Sophocleous and Aymen I. Idris
Description of the technology
The technology provides male and female rodent models of osteoporosis. The description of models, methods of performing the surgical procedures for their creation, and choice of reliable and safe anaesthetics are presented in form of protocol. The latter is also discussed
Ovariectomy in rodents is a procedure where ovaries are surgically excised. Hormonal changes resulting from ovary removal lead to an
This protocol also provides a detailed description of orchidectomy, a model for
The advantage of the technology is that it provides sufficiently comprehensive guide for creation of osteoporosis models in laboratory rodents, taking into account international practice and reminder that all animal procedures must conducted under control of regulatory ethics committees.
Practical application
Technology gives an important laboratory protocol for creation of osteoporosis models to use in experiments for study of this disease, investigations of aging processes, as well as drug discovery for osteoporosis treatment, search new perspective geroprotectors, etc.
Laboratories
- Rheumatic Diseases Unit, MRC Institute of Genetics and Molecular Medicine, Centre for Genomic and Experimental Medicine, Western General Hospital, University of Edinburgh, Edinburgh (UK)
Muscular-skeletal pharmacology, Academic Unit of Bone Biology, Mellanby Centre for Bone Research, Department of human metabolism, University of Sheffield, Sheffield (UK)
Links
http://www.nature.com/bonekeyreports/2014/141210/bonekey2014109/full/bonekey2014109.htmlPublications
- Sophocleous, A. & Idris, A.I. «Rodent models of osteoporosis." 3 BoneKEy Reports (2014): 614.
- Idris AI. «Ovariectomy/orchidectomy in rodents." In: Helfrich MH, Ralston SH (eds). Bone Research Protocols, Methods in Molecular Biology. Humana Press: New York, 2012, pp 545–551.
- Sophocleous A, et al. «The type 2 cannabinoid receptor regulates bone mass and
ovariectomy-induced bone loss by affecting osteoblast differentiation and bone formation." 152 Endocrinology (2011): 2141–2149.