Laure C. Martine, Boris M. Holzapfel, Anna
Description of the technology
Current in vivo models for investigating human primary bone tumors and cancer metastasis to the bone rely on the injection of human cancer cells into the mouse skeleton. This approach does not mimic
This technology developed a protocol to engineer humanized bone within immunodeficient hosts, which can be adapted to study the interactions between human cancer cells and a humanized bone microenvironment in vivo. A researcher trained in the principles of tissue engineering will be able to execute the protocol and yield study results within 4–6 months.
Additive biomanufactured scaffolds seeded and cultured with human
The most obvious application of this technology is to exploit the humanized bone environment as a preclinical in vivo model to research
Besides establishing a model for fundamental cancer biology research, the objective of this protocol is to use the human
- Queensland University of Technology (QUT), Brisbane, Queensland (Australia)
- Orthopedic Center for Musculoskeletal Research, University of Wuerzburg, Wuerzburg (Germany)
- George W Woodruff School of Mechanical Engineering, Georgia Institute of Technology, Atlanta (USA)
- Institute for Advanced Study, Technical University Munich, Garching (Germany)
- Martine, L.M. et al. «Engineering a humanized bone organ model in mice to study bone metastases." 12 Nature Protocols (2017): 639–663.
v. M.C.et al. «Differential osteogenicity of multiple donor-derivedhuman mesenchymal stem cells and osteoblasts in monolayer, scaffold-based3D culture and in vivo." 61 Biomed. Tech. (2016): 253–266.
- Thibaudeau, L. et al. «New mechanistic insights of integrin β1 in breast cancer bone colonization." 6 Oncotarget (2015): 332–344.