Dr. Shalini Prasad (right), professor of bioengineering at UT Dallas, and doctoral student Rujuta Munje designed a wearable, flexible biosensor that can reliably detect and quantify glucose from very small amounts of human sweat.
In a study recently published online in the journal Sensors and Actuators B: Chemical, Dr. Shalini Prasad, professor of bioengineering in the Erik Jonsson School of Engineering and Computer Science, and her
The team has previously demonstrated that their technology can detect cortisol in perspiration.
But for diabetics and those at risk for diabetes,
The flexible biosensor can reliably detect and quantify glucose in very small amounts of human sweat.
Typical
«In our sensor mechanism, we use the same chemistry and enzymatic reaction that are incorporated into blood glucose testing strips," Prasad said. «But in our design, we had to account for the low volume of ambient sweat that would be present in areas such as under a watch or wrist device, or under a patch that lies next to the skin.»
Prasad said that researchers who work with sweat often use a process called iontophoresis, which sends an electric current through the skin to generate enough perspiration for sensing experiments. However, because this method can lead to rashes and burns on the skin, the team sought an alternative that would work with small amounts of sweat.
Their design works with volumes of sweat less than a microliter, which is the approximate amount of liquid that would fit in a cube the size of a salt crystal.
The technology also provides a
Prasad and bioengineering doctoral student Rujuta Munje, lead author of the journal article, incorporated an
«We used known properties of textiles and weaves in our design," Prasad said. «What was innovative was the way we incorporated and positioned the electrodes onto this textile in such a way that allows a very small volume of sweat to spread effectively through the surface.»
Typical blood glucose testing strips also contain a molecule that ultimately amplifies the signal from the chemical reactions on the strip enough to register electronically on a monitoring device. But if used in a device that is worn next to the skin, those molecules can be irritating, Prasad said, which presented another challenge.
To ensure that such a tiny amount of sweat would generate a strong enough signal, Prasad and Munje modified the surface topography of the textile material.
«Our modifications allow this material to entrap glucose oxidase molecules, which effectively amplifies the signal," Prasad said. «We did it this way because we are thinking about possible commercialization — to make these, we need a fabrication process that is not complex.»
Prasad and Munje also were able to account for the fact that the chemistry of a person’s sweat changes throughout the day.
«Glucose is a tricky molecule to monitor because other factors can confound a signal," Prasad said. «For example, the pH, or acidity, of your sweat can vary greatly depending on the circumstances.»
She noted that when individuals exercise or are under stress, the level of other compounds in their sweat, such as cortisol and lactic acid, change as well, and these can interfere with glucose detection.
«We have shown that with our technology, we address three critical issues: low volume of ambient sweat, interference from other compounds and pH swings," Prasad said
Prasad and Munje tested their prototype using samples of human sweat from donors.
While a consumer product based on the technology is still a few years away, the concept was developed with commercialization and
«At this point, we are thinking of this sensor as something you use for a day and toss out, and we believe it could easily be incorporated into existing consumer electronics platforms," Prasad said. «We’re very excited about the potential for licensing this technology.»
The research was supported by the Cecil H. and Ida Green endowed fellowship at UT Dallas.
Researchers at UT Dallas and elsewhere have investigated whether glucose found in other bodily fluids — such as urine and tears — might be used to track glucose levels, further eliminating the need for invasive blood draws. Google, for example, is investigating a smart contact lens designed to measure glucose levels in tears.
