Description of the technology
This technology provides the isolation, culture and staining of primary osteoblasts from samples collected from organism of neonatal rodents and human. The calvaria and
Human osteoblasts, taken from several anatomical sources according to this technology, can be used to study osteoblast physiology. Human cells obtained from explant bone tissues consisting of a heterogeneous cell population that predominantly includes early osteoblasts, can be differentiated in vitro into mature,
The use of human osteoblasts will allow cell function to be investigated in patients suffering from
The technology proposes methods for the isolation of rat calvarial and
These methods could be also adapted to successfully culture mouse osteoblasts, which require more nutritional support in vitro.
Finally, the technology gives a protocol, describing how to culture primary human osteoblasts from bone samples.
- Department of Orthopaedic Surgery, Stanford University School of Medicine, Stanford (USA)
- Department of Surgery and Cancer, Imperial College London, London (UK)
- Department of Comparative Biomedical Sciences, Royal Veterinary College, London (UK)
- Taylor, S.E.B. et al. «Generation of rodent and human osteoblasts." 3 BoneKEy Reports (2014): 585.
- Orriss, I.R., Taylor, S.E., Arnett, T.R. «Rat osteoblast cultures." 816 Methods Mol Biol (2012): 31–41.
- Orriss, I.R. et al. «Optimisation of the differing conditions required for bone formation in vitro by primary osteoblasts from mice and rats." 34 Int J Mol Med (2014): 1201–1208.