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BioSens-AllTM: a platform to interrogate GPCR signaling

Description

Developers

I. Dorange, B. Breton, M. Coupal, M. Frauli, S. Schann.

Description of the technology

The BioSens-AllTM platform was implemented to interrogate the signaling complexity associated with GPCR activation. It consists of a combination of BRET-based biosensors each designed to follow one specific signaling event upon GPCR activation.

1. In a microplate, cells are co-transfected with the vector expressing the GPCR native sequence (no tag required) and the vectors for the expression of the desired biosensors.

2. Following protein-transient expression (48hrs after transfection), compounds are incubated with the cells and the activity of the biosensor is measured.

BioSens-AllTM platform offers a flexible service. With over 25 biosensors, the BioSens-AllTM platform constitutes a unique offering of integrated functional assays dedicated to the characterization of GPCR signaling. BioSens-AllTM enables the design of tailored studies (number and type of biosensors) reflecting investigators’ needs. Intracellular events measured within the

  BioSens-AllTM services include:

  • activation of protein Gαs, Gαi1, Gαi2, Gαi3, GαoA, GαoB, Gαz, Gα12, Gα13, Gαq, Gα11, Gα14, and Gα15/16,
  • recruitment of β-arrestin 1 and 2,
  • production of cAMP and calcium,
  • activity of effectors TPR1, GRK2/3, PKC, p63, Merlin, and p115.

Advantages of BioSens-AllTM studies:
  • will rapidly deliver the signaling signature for the GPCR of choice (example: wild type, mutant or orthologous),
  • no assay system bias (all cellular events measured with the same platform),
  • no structural modification of the receptor.

Practical application

This technology is dedicatied to drug discovery. BioSens-AllTM applications include but are not limited to:
  • orthosteric and allosteric compound bias effects,
  • signaling signature of wild-type receptors versus mutant variants,
  • orphan receptor signaling signature determination (deorphanization),
  • detection of receptor constitutive activity.

Laboratories

Domain Therapeutics, Neomed Institute, Montreal, Quebec, (Canada)

Links

http://www.domaintherapeutics.com/research-and-technology/biosens-all.html

Publications

Dorange, Ismet, et al. «BioSens-All ™: A new technology dedicated to GPCR biased ligand drug discovery." ABSTRACTS OF PAPERS OF THE AMERICAN CHEMICAL SOCIETY. Vol. 248. 1155 16TH ST, NW, WASHINGTON, DC 20036 USA: AMER CHEMICAL SOC, 2014.
Franchet, Christel, and Ismet Dorange. «GPCR binding technologies: an overview." Current topics in medicinal chemistry 15.24 (2015): 2476–2483.